Proteowa is an Australian biotechnology company focused on the recombinant protein expression and research in E. coli. We are the first and currently the only commercial institution in Western Australia that is actively working on the expression, refolding and formulation of recombinant proteins.
Located at the State Agricultural Biotechnology Centre (SABC) in Murdoch University in Western Australia, we have access to the complete range of high-tech equipment at SABC that is instrumental to our productivity and research capabilities. Our key proficiencies are as follows:
Built on our past experience in pharmaceutical protein manufacturing in academic and industrial environments, we offer contract expression of recombinant proteins for research and small industrial applications. The time required for our routine expression experiment is often 6-10 weeks but may be significantly longer if, for example, your protein is toxic to E. coli. We use synthetic (animal-free) media for all our expression experiments and often obtain 1 - 20 mg pure protein from the bench-top setups and up to 1 g from the fermenters. For the proteins toxic to E. coli, we've been able to use a propriety technique that results in reduced but still successful expressions of the proteins (often 1/3 to 1/10 of the comparable non-toxic proteins).
We own our proprietary technology to optimise the expression of recombinant proteins in E. coli. This is partly explained in our patents and a recent publication. To date our rate of success in protein expression is an immaculate 100%! If you think your protein is specially challenging, please talk to us about our guaranteed expression scheme (terms and conditions apply).
When required, we can also remove the endotoxin content of the proteins, for example to make it suitable for antibody production. However, please note that we are not currently GxP-certified and so the proteins that we express may not be tested/administered on humans, or used in medical and veterinary procedures.
Finally, if your protein requires downstream processing (including refolding, stabilisation/formulation, and lyophilisation) you may be also interested in our downstream contract research, explained in the next point.
We have extensive experience in the refolding of proteins that are otherwise expressed as inclusion bodies (IBs) in E. coli. We also use a verities of analytical techniques to investigate the best buffer compositions (formulation) to stabilise and lyophilise the proteins. Our key capabilities for this service include:
This includes mass spectrometry (eg. protein sequencing/identification, disulfide mapping, intact mass & adduct analysis, ...); thermal shift assay; dynamic light scattering analysis; chromatographic techniques (HPLC, FPLC); electrophoresis; etc.
IBs are refolded using methods guided by a combination of the above analytical techniques. Examples of the proteins that we folded with excellent success include various chemokines, cytokines and (cysteine-rich) IGF-binding proteins.
III. Stabilisation and formulation
Occasionally folded proteins (formed either naturally or by in vitro refolding) are unstable, leading to self-association, aggregation and precipitation. Alternatively you may need to identify the correct excipients that enable your proteins to withstand the lyophilisation and subsequent rehydration. Using suitable analytical techniques, we monitor the protein’s stability in various buffers and in the presence of additives to arrive at the best composition for these applications.
Besides contract research projects, we also offer scientific consultation for protein-related projects. Examples of our consultation projects include projects on the optimisation of protein expression and purification for clients in Queensland (Australia) and in Germany. In another example, we were involved in a peptidomics research project aimed at the comparative analysis of the peptide profiles obtained from the blood of animals under healthy and diseased conditions, and possible identification of peptide-based molecular markers.
For more information please see our service flyer.