While grassroot methods such as two-hybrid screening and affinity tags continue to be used as the initial step in many present-day studies of biomolecular interactions, an increasing number of modern laboratories are adopting label-free platforms like Bio-Layer Interferometry (BLI). These techniques not only offer greater sensitivity and speed but also provide real-time data on interactions.
At the heart of a BLI instrument lies a spectrometer that analyses the interference pattern…
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